Journal: Clinical & Experimental Metastasis

Article Title: Enhancement of metastatic ability by ectopic expression of ST6GalNAcI on a gastric cancer cell line in a mouse model

doi: 10.1007/s10585-011-9445-1

Figure Lengend Snippet: Effects of B72.3 administration on metastatic ability and survival of the recipient mice. a GFP-fluorescence view of peritoneal metastasis. Intraperitoneal dissemination such as peri-bile duct invasion ( arrow ), a cause of jaundice was reduced by B72.3 treatment. b Metastatic tumor weights excised from control mouse IgG-treated and B72.3-treated mice. c The incidence of jaundice was significantly lower in B72.3-treated mice than in IgG-treated control. * P < 0.05. d Overall survival of the GCIY/6L-transplanted recipient mice as depicted by the Kaplan-Meier method. The recipients administered with B72.3 ( open circles ) survived significantly longer than those with control mouse IgG ( closed circles ). P < 0.05; n = 6

Article Snippet: Three days after transfection, cells were harvested and stained for sTn with anti-sTn mAb, B72.3, and allophycocyanin (APC)-labeled goat anti-mouse Ig (BD Biosciences, San Diego, CA).

Techniques: Fluorescence

Journal: Clinical & Experimental Metastasis

Article Title: Enhancement of metastatic ability by ectopic expression of ST6GalNAcI on a gastric cancer cell line in a mouse model

doi: 10.1007/s10585-011-9445-1

Figure Lengend Snippet: Identification of sTn carrier proteins. a Cell lysates and culture supernatants of GCIY/6L (L) and GCIY/6S (S) were immunoprecipitated with B72.3 and analyzed by western blot with the same mAb. b The blot used in ( a ) was stripped and re-probed with anti-MUC1 mAb ( left ) or anti-CD44 polyclonal antibody ( right ). c Cell lysates and culture supernatants of GCIY/6L (L) and GCIY/6S (S) were immunoprecipitated with anti-MUC1 mAb ( left ) or anti-CD44 polyclonal antibody, and analyzed by western blot using B72.3

Article Snippet: Three days after transfection, cells were harvested and stained for sTn with anti-sTn mAb, B72.3, and allophycocyanin (APC)-labeled goat anti-mouse Ig (BD Biosciences, San Diego, CA).

Techniques: Immunoprecipitation, Western Blot

Journal: Clinical & Experimental Metastasis

Article Title: Enhancement of metastatic ability by ectopic expression of ST6GalNAcI on a gastric cancer cell line in a mouse model

doi: 10.1007/s10585-011-9445-1

Figure Lengend Snippet: Immunoprecipitation-western blot analysis of human gastric cancer tissue lysates for sTn-carrying MUC1. a Lysates from 4 advanced gastric cancer tissues were immunoprecipitated with B72.3 and subjected to western blot analysis with the same mAb ( left ), then re-probed with anti-MUC1 mAb ( right ). b The same lysates were immunoprecipitated with anti-MUC1 mAb and subjected to western blot analysis using B72.3 ( left ), then re-probed with anti-MUC1 mAb ( right )

Article Snippet: Three days after transfection, cells were harvested and stained for sTn with anti-sTn mAb, B72.3, and allophycocyanin (APC)-labeled goat anti-mouse Ig (BD Biosciences, San Diego, CA).

Techniques: Immunoprecipitation, Western Blot